Diabetes Impairs Synaptic Plasticity in the Superior Cervical Ganglion: Possible Role for BDNF and Oxidative Stress

被引:0
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作者
K. H. Alzoubi
O. F. Khabour
I. A. Alhaidar
A. M. Aleisa
K. A. Alkadhi
机构
[1] Jordan University of Science and Technology,Faculty of Pharmacy, Department of Clinical Pharmacy
[2] Jordan University of Science and Technology,Faculty of Applied Medical Sciences, Department of Medical Laboratory Sciences
[3] University of Houston,Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy
[4] King Saud University,Department of Pharmacology, College of Pharmacy
来源
Journal of Molecular Neuroscience | 2013年 / 51卷
关键词
BDNF; Oxidative stress; LTP; LTD; Basal synaptic transmission; PTP;
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摘要
The majority of diabetics develop serious disorders of the autonomic nervous system; however, there is no clear understanding on the causes of these complications. In this study, we examined the effect of streptozocin (STZ)-induced diabetes on activity-dependent synaptic plasticity, associated levels of brain-derived neurotrophic factor (BDNF) and antioxidant biomarkers in the rat sympathetic superior cervical ganglion. Diabetes (STZ-induced) was achieved by a single intraperitoneal injection of streptozocin (55 mg/kg).Compound action potentials were recorded from isolated ganglia before (basal) and after repetitive stimulation, or trains of paired pulses to express ganglionic long-term potentiation (gLTP) or long-term depression (gLTD). The input/output curves of ganglia from STZ-treated animals showed a marked rightward shift along most stimulus intensities, compared to those of ganglia from control animals, indicating impaired basal synaptic transmission in ganglia from STZ-induced diabetic animals. Repetitive stimulation induced robust gLTP and gLTD in ganglia isolated from control animals; the same protocols failed to induce gLTP or gLTD in ganglia from STZ-induced diabetic animals, indicating impairment of activity-dependent synaptic plasticity in these animals. Molecular analysis revealed significant reduction in the levels of BDNF and the ratio of glutathione/oxidized glutathione. Additionally, the activity of glutathione peroxidase, glutathione reductase, catalase, and the levels of thiobarbituric acid-reactive substances were increased in ganglia from STZ-treated animals. In conclusion, impaired basal synaptic transmission and synaptic plasticity are associated with reduced BDNF and altered oxidative stress biomarkers in the sympathetic ganglia from STZ-induced diabetic animals, suggesting a possible correlation of these factors with the manifestations of STZ-induced diabetes in the peripheral nervous system.
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页码:763 / 770
页数:7
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