Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling

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作者：
Jose Maria Carvajal-Gonzalez
Angel-Carlos Roman
Marek Mlodzik
机构：
[1] Tisch Cancer Institute,Department of Developmental & Regenerative Biology
[2] and Graduate School of Biomedical Sciences,undefined
[3] Icahn School of Medicine at Mount Sinai,undefined
[4] Champalimaud Neuroscience Programme,undefined
[5] Present address: Departamento de Bioquímica y Biología Molecular,undefined
[6] Facultad de Ciencias,undefined
[7] Universidad de Extremadura,undefined
[8] Badajoz 06071,undefined
[9] Spain.,undefined
来源：
Nature Communications | / 7卷
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摘要：
Planar cell polarity (PCP) signalling is a well-conserved developmental pathway regulating cellular orientation during development. An evolutionarily conserved pathway readout is not established and, moreover, it is thought that PCP mediated cellular responses are tissue-specific. A key PCP function in vertebrates is to regulate coordinated centriole/cilia positioning, a function that has not been associated with PCP in Drosophila. Here we report instructive input of Frizzled-PCP (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings. We show that centrioles are polarized in pupal wing cells as a readout of PCP signalling, with both gain and loss-of-function Fz/PCP signalling affecting centriole polarization. Importantly, loss or gain of centrioles does not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likely downstream of PCP-regulated actin polymerization. Together with vertebrate data, these results suggest a unifying model of centriole/cilia positioning as a common downstream effect of PCP signalling from flies to mammals.
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