The Use of ToF-SIMS for Analysis of Bioorganic Samples

被引：4
作者：
Pogorelov A.G. [1 ]
Gulin A.A. [2 ,3 ]
Pogorelova V.N. [1 ]
Panait A.I. [1 ]
Pogorelova M.A. [1 ]
Nadtochenko V.A. [2 ,3 ,4 ]
机构：
[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast
[2] Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow
[3] Department of Chemistry, Moscow State University, Moscow
[4] Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast
来源：
Biophysics | 2018年 / 63卷 / 2期
基金：
俄罗斯科学基金会;
关键词：
GV mice oocyte; molecule-specific imaging; scanning electron microscopy (SEM); spatial resolution; ToF-SIMS;
D O I：
10.1134/S0006350918020197
中图分类号：
学科分类号：
摘要：
The technique for the preparation of biological tissue sections developed for Electron Probe Microanalysis has been adapted for ToF-SIMS analysis of mouse GV stage oocytes. GV-oocyte sample preparation involves the following steps: plunge freezing, freeze drying, impregnation in an embedding medium, and section cutting. Molecule-specific images of the distribution of molecules in a single oocyte have been obtained with the described technique and ToF-SIMS analysis. The ToF-SIMS analysis data show that the efficient lateral image resolution is approximately 1 μm. Hence, ToF-SIMS enables us to study the distribution of chemical substances in relation to the morphological data obtained by scanning electron microscopy or conventional light microscopy. © 2018, Pleiades Publishing, Inc.
引用
收藏
页码：215 / 221
页数：6
相关论文
共 35 条
  • [1] Touboul D., Kollmer F., Niehuis E., Et al., J. Am. Soc. Mass Spectrom., 16, (2005)
  • [2] Vickerman J., Winograd N., Cluster Secondary Ion Mass Spectrometry: Principles and Applications, pp. 269-310, (2013)
  • [3] Breitenstein D., Hagenhoff B., Schnieders A., Surface Analysis and Techniques in Biology, pp. 37-70, (2014)
  • [4] Mahoney C.M., Mass Spectrom. Rev., 29, (2010)
  • [5] Mass Spectrometry Imaging: Principles and Protocols, (2010)
  • [6] Bobrowska J., Pabijan J., Wiltowska-Zuber J., Et al., Anal. Biochem., 511, (2016)
  • [7] Robinson M., Castner D.G., Biointerphases, 8, (2013)
  • [8] Schaepe K., Kokesch-Himmelreich J., Rohnke M., Et al., Biointerphases, 10, (2015)
  • [9] Luft M.D., J. Biophys. Biochem. Cytol., 9, (1961)
  • [10] Biological Specimen Preparation for Transmission Electron Microscopy, Practical Methods in Electron Microscopy, 17, (1998)
1234