The Use of ToF-SIMS for Analysis of Bioorganic Samples

被引：4

作者：

Pogorelov A.G. ^{[1
]}

Gulin A.A. ^{[2
,3
]}

Pogorelova V.N. ^{[1
]}

Panait A.I. ^{[1
]}

Pogorelova M.A. ^{[1
]}

Nadtochenko V.A. ^{[2
,3
,4
]}

机构：

[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast

[2] Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow

[3] Department of Chemistry, Moscow State University, Moscow

[4] Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast

来源：

Biophysics | 2018年 / 63卷 / 2期

基金：

俄罗斯科学基金会;

关键词：

GV mice oocyte; molecule-specific imaging; scanning electron microscopy (SEM); spatial resolution; ToF-SIMS;

D O I：

10.1134/S0006350918020197

中图分类号：

学科分类号：

摘要：

The technique for the preparation of biological tissue sections developed for Electron Probe Microanalysis has been adapted for ToF-SIMS analysis of mouse GV stage oocytes. GV-oocyte sample preparation involves the following steps: plunge freezing, freeze drying, impregnation in an embedding medium, and section cutting. Molecule-specific images of the distribution of molecules in a single oocyte have been obtained with the described technique and ToF-SIMS analysis. The ToF-SIMS analysis data show that the efficient lateral image resolution is approximately 1 μm. Hence, ToF-SIMS enables us to study the distribution of chemical substances in relation to the morphological data obtained by scanning electron microscopy or conventional light microscopy. © 2018, Pleiades Publishing, Inc.

引用

页码：215 / 221

页数：6

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