Single-molecule localization microscopy with microbial samples

被引：0
|
作者：
Feddersen H. [1 ]
Shin J.Y. [2 ,4 ]
Bramkamp M. [1 ,3 ]
机构：
[1] Fakultät Biologie, LMU München, München
[2] Max-Planck-Institut für Biochemie, Planegg-Martinsried
[3] Fakultät Biologie, Ludwig-Maximilians-Universität München, Großhaderner Straße 2–4, Planegg-Martinsried
[4] Max-Planck-Institut für Biochemie, Am Klopferspitz 18, Planegg-Martinsried
来源：
BIOspektrum | 2019年 / 25卷 / 2期
关键词：
D O I：
10.1007/s12268-019-1027-9
中图分类号：
学科分类号：
摘要：
Super-resolution microscopy visualizes cellular components near their macromolecular scale (~ 20 nm) and allows one to access structural and functional information within cells. This microscopy has been particularly valuable to unveil new biological insights in the field of bacterial cell biology, where the tiny size of bacterial cells imposed long-standing challenges to visualize cellular components. Here, we report a workflow to image bacterial cellular components with photoactivated localization microscopy that involves experimental design, sample preparation, data acquisition and analysis. © 2019, Springer-Verlag.
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页码：170 / 173
页数：3
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