Proline Accumulation in Transgenic Tobacco as a Result of Expression of Arabidopsis Δ1-Pyrroline-5-carboxylate synthetase (P5CS) During Osmotic Stress

被引：0
作者：
A. Yamchi
F. Rastgar Jazii
A. Mousavi
A. A. Karkhane
机构：
[1] National Institute of Genetic Engineering and Biotechnology (NIGEB),Department of Plant Biotechnology, Faculty of Agriculture
[2] Isfahan University of Technology,Division of Plant Pathology
[3] Indian Agricultural Research Institute,undefined
来源：
Journal of Plant Biochemistry and Biotechnology | 2007年 / 16卷
关键词：
cDNA cloning; Δ; -Pyrroline-5-carboxylate synthetase; osmotic stress; drought tolerance; proline; tobacco transformation;
D O I：
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中图分类号：
学科分类号：
摘要：
The entire coding sequence of the bi-functional enzyme, Δ1-Pyrroline-5-carboxylate synthetase (P5CS) from Arabidopsis thaliana was reverse-transcribed, amplified and expressed under the control of CaMV 35S promoter in transgenic tobacco plants. Several lines were established and tested for the expression of P5CS. Drought and salinity were applied as osmotic stresses and proline content of the transformed plants was compared with that of non-transformed controls. Results indicate that transgenic lines express higher levels of proline and show enhanced resistance to the applied osmotic stress as compared to the non-transgenic plants.
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页码：9 / 15
页数：6
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