Detection of porcine reproductive and respiratory syndrome virus in the ovary of gilts culled due to reproductive disturbances

被引：1
作者：
Tummaruk P. [1 ]
Phoophitphong D. [1 ]
Olanratmanee E.-O. [2 ]
Thanawongnuwech R. [3 ]
机构：
[1] Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok
[2] Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Chonburi
[3] Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok
来源：
Comparative Clinical Pathology | 2015年 / 24卷 / 4期
关键词：
Immunohistochemistry; Ovary; Pig; PRRSV; Reproduction;
D O I：
10.1007/s00580-014-2006-7
中图分类号：
学科分类号：
摘要：
Replacement gilts are a major source of introducing the porcine reproductive and respiratory syndrome (PRRS) virus into the swine breeding herd. The objective of the present study was to determine the prevalence of PRRS virus antigen by detection in the ovary of gilts culled due to reproductive disturbances. Ovarian tissues were collected from 100 Landrace x Yorkshire crossbred slaughtered gilts in Thailand. PRRS virus antigen in the ovarian tissues were detected by using immunohistochemistry. On average, the gilts were culled at 277 days of age at a body weight of 148 kg. Of these gilts, 75 had attained puberty and 25 had not attained puberty. PRRS virus antigens were detected in the macrophages within the ovary. The histological morphology of macrophages containing the PRRS virus in ovarian tissue was similar to that found in the lung tissue. Of all the ovarian tissues (n = 100), the PRRS virus was detected in 70 (70.0 %) ovaries. The detection frequency of the PRRS virus in the ovarian tissue of gilts did not differ significantly among the culling reasons (P = 0.496). The gilts that had exhibited standing oestrus had a lower PRRS virus detection than those that had never exhibited standing oestrus (62.8 versus 92.0 %, respectively, P = 0.005). Gilts with a body weight of 151–160 kg had a higher frequency of PRRS virus detection (89.5 %) than gilts with a body weight of ≤130 kg (52.9 %, P = 0.025) and 131–140 kg (50.0 %, P = 0.032). The ovulation rate in the ovary containing the PRRS virus tended to be lower than the ovary without the PRRS virus (14.8 and 16.7 corpora lutea (CL), respectively, P = 0.196). These findings indicated that the natural exposure to PRRS virus in the replacement gilts caused an existence of the virus in the ovarian tissues and may subsequently influence the ovarian function. © 2014, Springer-Verlag London.
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页码：903 / 910
页数：7
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共 22 条
  • [1] Araki M., Fukumatsu Y., Katabuchi H., Shultz L.D., Takahashi K., Okamura H., Follicular development and ovulation in macrophage colony-stimulating factor-deficient mice homozygous for the osteopetrosis (op) mutation, Biol Reprod, 54, pp. 478-484, (1996)
  • [2] Benson J.E., Yaeger J.M., Ford P.S., Effect of porcine reproductive and respiratory syndrome virus infection on the ovary and progesterone levels in third trimester gestation sows, Theriogenology, 56, pp. 777-785, (2001)
  • [3] Damrongwatanapokin S., Arsayuth K., Kongkrong C., Parchariyanon S., Pinyochon W., Tantaswasdi U., Serological studies and isolation of porcine reproductive and respiratory syndrome (PRRS) virus in Thailand, J Thai Vet Med Assoc, 47, pp. 19-31, (1996)
  • [4] Diaz I., Gimeno M., Callen A., Pujols J., Lopez S., Charreyre C., Joisel F., Mateu E., Comparison of different vaccination schedules for sustaining the immune response against porcine reproductive and respiratory syndrome virus, Vet J, 197, pp. 438-444, (2013)
  • [5] Guerin B., Pozzi N., Virus in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination, Theriogenology, 63, pp. 556-572, (2005)
  • [6] Keffaber K.K., Reproduction failure of unknown etiology, Am Assoc Swine Pract Newsl, 1, (1989)
  • [7] Nilubol D., Tripipat T., Hoonsuwan T., Tipsombatboon P., Piriyapongsa J., Dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 following modified live PRRSV vaccination in a PRRSV-infected herd, Arch Virol, 159, pp. 17-27, (2014)
  • [8] Olanratmanee E., Nuntawan Na Ayudhya S., Thanawongnuwech R., Kunavongkrit A., Tummaruk P., Reproductive parameters following a PRRS outbreak where a whole-herd PRRS MLV vaccination strategy was instituted post-outbreak, Trop Anim Health Prod, 45, pp. 1099-1106, (2013)
  • [9] Olanratmanee E., Wangnaitham S., Thanawongnuwech R., Kunavongkrit A., Tummaruk P., Prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterus tissue in gilts culled due to reproductive disturbance in Thailand, Trop Anim Health Prod, 43, pp. 451-457, (2011)
  • [10] Orisaka M., Tajima K., Tsang B.K., Kotsuji F., Oocyte-granulosa-theca cell interactions during preantral follicular development, J Ovarian Res, 2, pp. 9-15, (2009)
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