Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer

被引：0
作者：
Zhenfang Wu
Zhiqian Xu
Xian Zou
Fang Zeng
Junsong Shi
Dewu Liu
Johann Urschitz
Stefan Moisyadi
Zicong Li
机构：
[1] South China Agricultural University,Department of Animal Genetics, Breeding and Reproduction
[2] South China Agricultural University,Department of Animal Production, College of Animal Science
[3] Guangdong Wen’s Food Group Co. Ltd.,Wen’s Research Institute
[4] University of Hawaii at Manoa,Department of Anatomy, Biochemistry and Physiology, Institute for Biogenesis Research, John A. Burns School of Medicine
来源：
Transgenic Research | 2013年 / 22卷
关键词：
Transposon; Transposase; SCNT; Cloning; Transgenesis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2 % of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT.
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页码：1107 / 1118
页数：11
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