Clinical observation, acute phase protein levels, and histopathological changes of mammary gland in experimentally infected goats with Staphylococcus aureus

被引：1

作者：

Sadiq M.B. ^{[1
]}

Mansor R. ^{[1
]}

Syed-Hussain S.S. ^{[2
]}

Saharee A.A. ^{[2
]}

Zakaria Z. ^{[3
]}

Syahirah A.A. ^{[1
]}

Bousnane I. ^{[1
]}

Adlina Z.A.J. ^{[4
]}

Salleh A. ^{[5
]}

Sukri W.I.W.M. ^{[1
]}

Mustaffa-Kamal F. ^{[3
]}

Ramanoon S.Z. ^{[1
]}

机构：

[1] Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang

[2] Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang

[3] Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang

[4] Department of Pharmacology, University of Oxford, Oxford

[5] Department of Veterinary Laboratory Diagnostic, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang

来源：

Comparative Clinical Pathology | 2019年 / 28卷 / 4期

关键词：

Acute phase proteins; Goats; Haptoglobin; Histopathology; Mastitis; Serum amyloid A;

D O I：

10.1007/s00580-019-02926-x

中图分类号：

学科分类号：

摘要：

Staphylococcus aureus (S. aureus) is a major cause of mastitis in small ruminants, and studies on the potential application of acute phase proteins (APPs) in disease biomarkers and understanding the disease mechanism in mastitis affecting small ruminants are limited. Thus, the objective of this study was to determine clinical, histopathological changes and serum concentrations of serum amyloid A (SAA) and haptoglobin (Hp) associated with experimentally induced mastitis using S. aureus. Left mammary quarter in the does was challenged with sterile Ringers buffered salt solution as group 1 (control animals; n = 6) and S. aureus (1.5 × 108 cfu/ml) as group 2 (inoculated does; n = 5) respectively. The animals were monitored at various time intervals for the signs of mastitis and serum concentrations of Hp and SAA. Majority of the animals in the inoculated group showed typical signs of acute mastitis characterized with fever, warm, painful, and swollen mammary gland as early as 24-h post-inoculation (PI). There was significant increased (p < 0.05) in the levels of Hp and SAA in the serum of inoculated animals at 24-h post-inoculation. Histopathological changes in the mammary gland of the inoculated goats included generalized and severe infiltration of the interstitial spaces with polymorphonuclear neutrophils (PMN), severe necrosis of secretory cells, and hyperplastic epithelial and luminal lining. In conclusion, inoculation of S. aureus intramammarily leads to an increment of Hp and SAA at 24-h PI which indicates that both APPs played crucial role as biomarkers in detection and monitoring of S. aureus mastitis in goats. © 2019, Springer-Verlag London Ltd., part of Springer Nature.

引用

页码：1069 / 1075

页数：6

共 33 条

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