A monoclonal antibody against 47.2 kDa cell surface antigen prevents adherence and affects biofilm formation of Candida albicans

被引：0
作者：
Nripendra Nath Mishra
Shakir Ali
Praveen K. Shukla
机构：
[1] CSIR-Central Drug Research Institute,Medical Mycology Lab, Division of Microbiology
[2] Jamia Hamdard,Department of Biochemistry
来源：
World Journal of Microbiology and Biotechnology | 2015年 / 31卷
关键词：
Biofilm; Cell surface protein; Hela cells; Monoclonal antibody;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Candida albicans is an opportunistic dimorphic pathogen that exists in both planktonic and biofilm phases causing deep-rooted infections in mainly immunocompromised patients. Antibodies are believed to play anti-Candida activity by different mechanisms, like inhibition of adhesion and neutralization of virulence-related antigens. Inhibition of adhesion is one of the important strategies to prevent Candida infections and biofilm formation. In this study, monoclonal antibody (MAb 7D7) against C. albicans biofilm cell surface antigen (47.2 kDa) was generated to determine the changes in adherence and viability of C. albicans. In this regard XTT assay was carried out in 30, 60, 90 min and 48 h (maturation time) time points using MAb 7D7 and it (MAb 7D7) was found to be effective against adhesion and the formation of C. albicans biofilm on polystyrene as well as monolayer of human epithelial cells (HeLa). This result may also prove to be a valuable addition to the reagents available to study C. albicans cell surface dynamics and interaction of the fungus with host cells.
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页码：11 / 21
页数：10
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