One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability

被引：0
|
作者：
Anshu Gupta
Ipsita Roy
S.K. Khare
V.S. Bisaria
M.N. Gupta
机构：
[1] Indian Institute of Technology,Chemistry Department
[2] Delhi,Department of Biochemical Engineering and Biotechnology
[3] Hauz Khas,undefined
[4] Indian Institute of Technology,undefined
[5] Delhi,undefined
[6] Hauz Khas,undefined
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
enzyme thermostabilization; ethylene glycol; xylanase purification;
D O I：
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中图分类号：
学科分类号：
摘要：
Hydrophobic interaction chromatography with Phenyl Sepharose 6 Fast Flow resulted in 7-fold purification of xylanaseactivity from Melanocarpus albomyces with over 60% recovery of theactivity. The purified preparation consisted of two major isoenzymes out of seven present in the organism. Ethyleneglycol, used as the eluent, enhanced the thermal stability of the xylanase activity from a half-life of 2.2 h at 55 °C to almost 40 h.
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页码：2005 / 2009
页数：4
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