Surface display of lipolytic enzyme, Lipase A and Lipase B of Bacillus subtilis on the Bacillus subtilis spore

被引：0
|
作者：
Junehyung Kim
机构：
[1] Dong-A University,Department of Chemical Engineering
来源：
Biotechnology and Bioprocess Engineering | 2017年 / 22卷
关键词：
spore-display; lipase; enzyme stability; organic solvent;
D O I：
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学科分类号：
摘要：
For the enhancement of lipase stability in organic solvent containing reaction, live immobilization method, using Bacillus subtilis spore as a display vehicle was attempted. Bacillus subtilis coat protein cotE was used as an anchoring motif for the display of lipA and lipB of Bacillus subtilis. Using this motif, lipolytic enzyme Lipase A and Lipase B were functionally displayed on the surface of Bacillus subtilis spore. Purified spore displaying CotE-LipB fusion protein showed higher lipolytic activity compared to that of CotE-LipA fusion protein. The surface localization of Lipase B was verified with flow cytometry and protease accessibility experiment. Spore displayed lipase retained its activity against acetone and benzene which completely deactivated free soluble lipase in the same reaction condition.
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页码：462 / 468
页数：6
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