Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp. pekinensis)

被引：0
作者：
Xiaoyong Xu
Zeping Yang
Xilu Sun
Lugang Zhang
Zhiyuan Fang
机构：
[1] College of Horticulture,College of Horticulture
[2] State Key Laboratory of Crop Stress Biology in Arid Areas,Institute of Vegetables and Flowers
[3] Northwest A&F University,undefined
[4] Shanxi Agriculture University,undefined
[5] Chinese Academy of Agricultural Sciences,undefined
来源：
Acta Physiologiae Plantarum | 2014年 / 36卷
关键词：
Flower bud development; Heading Chinese cabbage; Male sterility; qRT-PCR; Reference gene selection;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The accuracy of quantitative real-time PCR (qRT-PCR) depends on the stability of the reference gene used for normalization. In heading Chinese cabbage (Brassica rapa L. ssp. pekinensis), the most stable reference genes for qRT-PCR during flower bud development have not been elucidated. In this study, the statistical software geNorm was used to test eight candidate reference genes during flower bud development in male sterile (Ms) and fertile (Mf) plants. The result revealed that the stability order was Tub/GAPDH > Cyp > EF1a > U34559 > BrTip41 > Apr > 18S rRNA, Tub and GAPDH were the most stable genes [average expression stability (M) 0.614], and the combined use of six reference genes [pairwise variation (V) 0.15] was suggested to be the optimal reference gene for qRT-PCR during flower bud development. Furthermore, the expressions of BcPME31 during flower bud development normalized with the combined use of six reference genes and with GAPDH or Tub alone were compared; the various results also suggested that selection of the optimal reference gene was necessary for gene expression analysis.
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页码：809 / 814
页数：5
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