A microfabricated fluorescence-activated cell sorter

被引：0
作者：
Anne Y. Fu
Charles Spence
Axel Scherer
Frances H. Arnold
Stephen R. Quake
机构：
[1] California Institute of Technology,Department of Applied Physics
[2] California Institute of Technology,Division of Chemistry and Chemical Engineering
来源：
Nature Biotechnology | 1999年 / 17卷
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摘要：
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (μFACS) for sorting various biological entities. Compared with conventional FACS machines, the μFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used μFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
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页码：1109 / 1111
页数：2
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