Immunolabeling artifacts and the need for live-cell imaging

被引：0
作者：
Ulrike Schnell
Freark Dijk
Klaas A Sjollema
Ben N G Giepmans
机构：
[1] Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen
来源：
Nature Methods | 2012年 / 9卷 / 2期
关键词：
D O I：
10.1038/nmeth.1855
中图分类号：
学科分类号：
摘要：
Fluorescent fusion proteins have revolutionized examination of proteins in living cells. Still, studies using these proteins are met with criticism because proteins are modified and ectopically expressed, in contrast to immunofluorescence studies. However, introducing immunoreagents inside cells can cause protein extraction or relocalization, not reflecting the in vivo situation. Here we discuss pitfalls of immunofluorescence labeling that often receive little attention and argue that immunostaining experiments in dead, permeabilized cells should be complemented with live-cell imaging when scrutinizing protein localization. © 2012 Nature America, Inc. All rights reserved.
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页码：152 / 158
页数：6
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