Potentiation of antifungal activity of amphotericin B by azithromycin againstAspergillus species

被引:0
作者
M. H. Nguyen
C. J. Clancy
Y. C. Yu
A. S. Lewin
机构
[1] University of Florida College of Medicine,Department of Medicine
[2] University of Florida College of Medicine,Department of Molecular Genetics
[3] VA Medical Center,Department of Medicine
来源
European Journal of Clinical Microbiology and Infectious Diseases | 1997年 / 16卷
关键词
Internal Medicine; Protein Synthesis; Methionine; Potential Role; Amphotericin;
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摘要
The potential role of azithromycin in combination with amphotericin B against 25 clinical isolates ofAspergillus was assessed. The MIC of amphotericin B was 1 μg/ml for 44% of the isolates, 0.5 μg/ml for 48%, and 0.25 μg/ml for 8%. All isolates were resistant to azithromycin. Synergism, defined as a ≥ twofold reduction in the MIC of both drugs upon combination, was demonstrated between amphotericin B and azithromycin for all 25 isolates. To prove that azithromycin exerts its antifungal effect by inhibiting protein synthesis, we studied [35S]-methionine incorporation into protein inone Aspergillus isolate. Neither amphotericin B at 0.125 μg/ml (fourfold below its MIC) nor azithromycin at 16 μg/ml (≥ 16-fold below its MIC) had any effect on protein synthesis when tested alone. Upon combination, however, a 68% inhibition in protein synthesis was evident by the inhibition of [35S]-methionine incorporation.
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页码:846 / 848
页数:2
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