Sensitive detection of miRNA based on enzyme-propelled multiple photoinduced electron transfer strategy

被引：7
作者：
Yang Y. [1 ]
Liu S. [2 ]
Cui X. [1 ]
Yang L. [1 ]
Zhang J. [3 ]
Mao X. [2 ,4 ]
Gao Y. [1 ]
机构：
[1] College of Chemistry and Chemical Engineering, Anqing Normal University, Anqing
[2] Key Laboratory of Aqueous Environment Protection and Pollution Control of Yangtze River in Anhui of Anhui Provincial Education Department, College of Resources and Environment, Anqing Normal University, Anqing
[3] State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia University, Yinchuan
[4] Laboratory of Crop Genetic Breeding Improvement, School of Life Sciences, Shanghai University, Shanghai
来源：
Microchimica Acta | 2021年 / 188卷 / 06期
基金：
中国国家自然科学基金;
关键词：
G-quadruplex; microRNA; Photoinduced electron transfer; Silver nanocluster; Terminal deoxynucleotidyl transferase;
D O I：
10.1007/s00604-021-04874-2
中图分类号：
学科分类号：
摘要：
A method is presented that uses photoinduced electron transfer (PET) for the determination of microRNAs (miRNAs) in clinical serum samples and complicated cell samples by using a smartphone. miRNA-21 is adopted as a model analyte. A 3′-phosphorylated DNA probe containing AgNCs is synthesized and hybridized with miRNA-21. Subsequently, the probe is cleaved specifically by duplex-specific nuclease to form 3′-hydroxylated products, then extended by terminal deoxynucleotidyl transferase (TdT) with superlong G for G-quadruplex/hemin units fabrication. In this way, PET occurred between AgNCs and produced G-quadruplex/hemin units, leading to the fluorescence quenching of AgNCs. Notably, the fluorescence images can be captured and translated into digital information by smartphone, resulting in a direct quantitative determination of miRNA. As a result, our strategy for miRNA assay is achieved with a satisfactory detection limit of 1.43 pM. Interestingly, TdT-propelled G-quadruplex/hemin units as multiple electron acceptors promote the sensitivity of miRNA monitoring. Different miRNAs assays are realized by adjusting the complimentary sequences of DNA probe. These qualities not only broaden the practical application of PET-based strategy, but also provide a new insight into the nucleic acid detection. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
引用
收藏
相关论文
共 43 条
  • [1] Calin G.A., Croce C.M., MicroRNA signatures in human cancers, Nat Rev Cancer, 6, pp. 857-866, (2006)
  • [2] Ramani V., Qiu R., Shendure J., High-throughput determination of RNA structure by proximity ligation, Nat Biotechnol, 33, pp. 980-984, (2015)
  • [3] Lu J., Getz G., Miska E.A., Alvarez-Saavedra E., Lamb J., Peck D., Sweet-Cordero A., Ebet B.L., Mak R.H., Ferrando A.A., Downing J.R., Jacks T., Horvitz H.R., Golub T.R., MicroRNA expression profiles classify human cancers, Nature, 435, pp. 834-838, (2005)
  • [4] Kumar M.S., Lu J., Mercer K.L., Golub T.R., Jacks T., Impaired microRNA processing enhances cellular transformation and tumorigenesis, Nat Genet, 39, pp. 673-677, (2007)
  • [5] Li S., Xu L.G., Ma W., Wu X.L., Sun M.Z., Kuang H., Wang L.B., Kotov N.A., Xu C.L., Dual-mode ultrasensitive quantification of microRNA in living cells by chiroplasmonic nanopyramids self-assembled from gold and upconversion nanoparticles, J Am Chem Soc, 138, pp. 306-312, (2016)
  • [6] Reddy K.B., MicroRNA(miRNA) in cancer, Cancer Cell Int, 15, (2015)
  • [7] Chandrasekaran A.R., MacIsaac M., Dey P., Levchenko O., Zhou L.F., Andres M., Dey B.K., Halvorsen K., Cellular microRNA detection with miRacles: microRNA activated, Sci Adv, 5, (2019)
  • [8] Kim S., Park J.E., Hwang W., Seo J., Lee Y.K., Hwang J.H., Nam J.M., Optokinetically encoded nanoprobe-based multiplexing strategy for microRNA profiling, J Am Chem Soc, 139, pp. 3558-3566, (2017)
  • [9] Varallyay E., Burgyan J., Havelda Z., MicroRNA detection by northern blotting using locked nucleic acid probes, Nature, 3, pp. 190-196, (2008)
  • [10] Lee J.M., Cho H., Jung Y., Fabrication of a structure-specific RNA binder for array detection of label-free microRNA, Angew Chem Int Edit, 49, pp. 8662-8665, (2010)
12345