Isolation and characterization of twelve polymorphic microsatellite loci in Polygonatum cyrtonema and cross-species amplification

被引:2
|
作者
Wen-juan Cheng
Ting-ting Liu
Hai-long Wu
Shou-biao Zhou
Shou-qin Xuan
Guo-ping Zhu
机构
[1] Anhui Normal University,Key Laboratory of Molecular Evolution and Biodiversity and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Science
来源
关键词
Microsatellite; Cross-amplification; Population genetics;
D O I
暂无
中图分类号
学科分类号
摘要
Twelve novel microsatellite loci were isolated in a perennial herb, Polygonatum cyrtonema. The polymorphism of each locus was analyzed by screening 23 individuals from a natural population. The number of alleles ranged from 2 to 11, and the observed and expected heterozygosities ranged from 0.273 to 0.727 and from 0.268 to 0.865, respectively. These microsatellite loci were cross-amplified for four congeneric species and nine loci can be amplified successfully in all four species. The results suggest that these microsatellite markers would contribute to the population genetic studies of P. cyrtonema and other related species.
引用
收藏
页码:105 / 107
页数:2
相关论文
共 50 条
  • [21] Isolation and characterization of microsatellite loci in Lesquerella fendleri (Brassicaceae) and cross-species amplification
    Salywon, A
    Dierig, DA
    MOLECULAR ECOLOGY NOTES, 2006, 6 (02): : 382 - 384
  • [22] Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification
    Kramer, Andrea T.
    Fant, Jeremie B.
    MOLECULAR ECOLOGY NOTES, 2007, 7 (06): : 998 - 1001
  • [23] ISOLATION, CHARACTERIZATION, AND CROSS-SPECIES AMPLIFICATION OF POLYMORPHIC MICROSATELLITE MARKERS FOR PILOSOCEREUS MACHRISII (CACTACEAE)
    Perez, Manolo F.
    Teo, Mariana F.
    Zappi, Daniela C.
    Taylor, Nigel P.
    Moraes, Evandro M.
    AMERICAN JOURNAL OF BOTANY, 2011, 98 (08) : E204 - E206
  • [24] Isolation and characterization of twenty-one polymorphic microsatellite loci from Schizothorax o’connori and cross-species amplification
    XIANG-ZHAO GUO
    GUI-RONG ZHANG
    KAI-JIAN WEI
    SHAN-SHAN GUO
    JIAN-HUI QIN
    Jonathan P. A. GARDNER
    Journal of Genetics, 2016, 93 : 60 - 64
  • [25] Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification
    Li, Long-na
    Zeng, Songjun
    Zheng, Feng
    Chen, Zhi-lin
    Wu, Kun-lin
    Zhang, Jian-xia
    Duan, June
    HORTSCIENCE, 2010, 45 (08) : 1286 - 1287
  • [26] Isolation and characterization of twenty-one polymorphic microsatellite loci from Schizothorax o'connori and cross-species amplification
    Guo, Xiang-Zhao
    Zhang, Gui-Rong
    Wei, Kai-Jian
    Guo, Shan-Shan
    Qin, Jian-Hui
    Gardner, Jonathan P. A.
    JOURNAL OF GENETICS, 2016, 93 : E60 - E64
  • [27] Isolation and characterization of microsatellite loci in Pseudoplatystoma corruscans (Siluriformes: Pimelodidae) and cross-species amplification
    Revaldaves, E
    Pereira, LHG
    Foresti, F
    Oliveira, C
    MOLECULAR ECOLOGY NOTES, 2005, 5 (03): : 463 - 465
  • [28] Characterization and cross-species amplification of microsatellite loci in a Cyprinodon species flock
    Strecker, Ulrike
    MOLECULAR ECOLOGY NOTES, 2006, 6 (03): : 843 - 846
  • [29] Isolation of eleven polymorphic microsatellite loci for the endangered Sillago parvisquamis and cross-species amplification with Sillago japonica
    Tetsuya Umino
    Kanako Ueno
    Takeshi Mihara
    Masaru Koike
    Makoto Watanabe
    Kamarudin Ahmad-Syazni
    Makoto Ishitani
    Kenichi Ohara
    Conservation Genetics Resources, 2013, 5 : 771 - 773
  • [30] Isolation of eleven polymorphic microsatellite loci for the endangered Sillago parvisquamis and cross-species amplification with Sillago japonica
    Umino, Tetsuya
    Ueno, Kanako
    Mihara, Takeshi
    Koike, Masaru
    Watanabe, Makoto
    Ahmad-Syazni, Kamarudin
    Ishitani, Makoto
    Ohara, Kenichi
    CONSERVATION GENETICS RESOURCES, 2013, 5 (03) : 771 - 773