Isolation and characterization of twelve polymorphic microsatellite loci in Polygonatum cyrtonema and cross-species amplification

被引：2
|
作者：
Wen-juan Cheng
Ting-ting Liu
Hai-long Wu
Shou-biao Zhou
Shou-qin Xuan
Guo-ping Zhu
机构：
[1] Anhui Normal University,Key Laboratory of Molecular Evolution and Biodiversity and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Science
来源：
Conservation Genetics Resources | 2010年 / 2卷
关键词：
Microsatellite; Cross-amplification; Population genetics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Twelve novel microsatellite loci were isolated in a perennial herb, Polygonatum cyrtonema. The polymorphism of each locus was analyzed by screening 23 individuals from a natural population. The number of alleles ranged from 2 to 11, and the observed and expected heterozygosities ranged from 0.273 to 0.727 and from 0.268 to 0.865, respectively. These microsatellite loci were cross-amplified for four congeneric species and nine loci can be amplified successfully in all four species. The results suggest that these microsatellite markers would contribute to the population genetic studies of P. cyrtonema and other related species.
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页码：105 / 107
页数：2
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