Gapless assembly of maize chromosomes using long-read technologies

被引：0

作者：

Jianing Liu

Arun S. Seetharam

Kapeel Chougule

Shujun Ou

Kyle W. Swentowsky

Jonathan I. Gent

Victor Llaca

Margaret R. Woodhouse

Nancy Manchanda

Gernot G. Presting

David A. Kudrna

Magdy Alabady

Candice N. Hirsch

Kevin A. Fengler

Doreen Ware

Todd P. Michael

Matthew B. Hufford

R. Kelly Dawe

机构：

[1] University of Georgia,Department of Genetics

[2] Iowa State University,Genome Informatics Facility

[3] Cold Spring Harbor Laboratory,Department of Ecology, Evolution, and Organismal Biology

[4] Iowa State University,Department of Plant Biology

[5] University of Georgia,Molecular Biosciences and Bioengineering

[6] Corteva Agriscience™,Arizona Genomics Institute, School of Plant Sciences

[7] USDA-ARS Corn Insects and Crop Genetics Research Unit,Georgia Genomics and Bioinformatics Core Laboratory

[8] University of Hawaii,Department of Agronomy and Plant Genetics

[9] University of Arizona,Informatics Department

[10] University of Georgia,undefined

[11] University of Minnesota,undefined

[12] USDA ARS NAA Robert W. Holley Center for Agriculture and Health,undefined

[13] Agricultural Research Service,undefined

[14] J. Craig Venter Institute,undefined

来源：

Genome Biology | / 21卷

关键词：

Gapless assembly; Maize genome; Knob structure; Meiotic drive; Long-read technology;

D O I：

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中图分类号：

学科分类号：

摘要：

Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. This genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), and 53 Mb of the Ab10 meiotic drive haplotype. The data also reveal the internal structure of seven centromeres and five heterochromatic knobs, showing that the major tandem repeat arrays (CentC, knob180, and TR-1) are discontinuous and frequently interspersed with retroelements.

引用

共 273 条

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