The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis

被引：19
作者：
Bodor A.M. [1 ]
Jänsch L. [2 ]
Wissing J. [2 ]
Wagner-Döbler I. [1 ]
机构：
[1] Helmholtz-Centre for Infection Research, Group Microbial Communication, Division of Microbial Pathogenesis, 38124 Braunschweig
[2] Helmholtz-Centre for Infection Research, Group Cellular Proteomics, Division of Microbial Pathogenesis, 38124 Braunschweig
来源：
BMC Research Notes | / 4卷 / 1期
关键词：
autoinducer-2; biofilm; luxS; quorum sensing; secretome; Shewanella oneidensis;
D O I：
10.1186/1756-0500-4-180
中图分类号：
学科分类号：
摘要：
Background: The luxS gene in Shewanella oneidensis was shown to encode an autoinducer-2 (AI-2)-like molecule, the postulated universal bacterial signal, but the impaired biofilm growth of a luxS deficient mutant could not be restored by AI-2, indicating it might not have a signalling role in this organism. Findings. Here, we provide further evidence regarding the metabolic role of a luxS mutation in S. oneidensis. We constructed a luxS mutant and compared its phenotype to a wild type control with respect to its ability to remove AI-2 from the medium, expression of secreted proteins and biofilm formation. We show that S. oneidensis has a cell-dependent mechanism by which AI-2 is depleted from the medium by uptake or degradation at the end of the exponential growth phase. As AI-2 depletion is equally active in the luxS mutant and thus does not require AI-2 as an inducer, it appears to be an unspecific mechanism suggesting that AI-2 for S. oneidensis is a metabolite which is imported under nutrient limitation. Secreted proteins were studied by iTraq labelling and liquid chromatography mass spectrometry (LC-MS) detection. Differences between wild type and mutant were small. Proteins related to flagellar and twitching motility were slightly up-regulated in the luxS mutant, in accordance with its loose biofilm structure. An enzyme related to cysteine metabolism was also up-regulated, probably compensating for the lack of the LuxS enzyme. The luxS mutant developed an undifferentiated, loosely-connected biofilm which covered the glass surface more homogenously than the wild type control, which formed compact aggregates with large voids in between. Conclusions: The data confirm the role of the LuxS enzyme for biofilm growth in S. oneidensis and make it unlikely that AI-2 has a signalling role in this organism. © 2011 Bodor et al; licensee BioMed Central Ltd.
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