Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

被引：33
作者：
Breda C.A. [1 ,2 ]
Gasperini A.M. [1 ,2 ]
Garcia V.L. [3 ]
Monteiro K.M. [4 ]
Bataglion G.A. [5 ]
Eberlin M.N. [5 ]
Duarte M.C.T. [2 ]
机构：
[1] School of Food Engineering, State University of Campinas, UNICAMP, Monteiro Lobato Street, 80, Barão Geraldo, Campinas, 13083-862, São Paulo
[2] Microbiology Division of Research Center for Chemistry, Biology and Agriculture – CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, 13148-218, São Paulo
[3] Organic Chemistry and Pharmaceutical Division of Research Center for Chemistry, Biology and Agriculture – CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, 13148-218, São Paulo
[4] Pharmacology and Toxicology Division of Research Center for Chemistry, Biology and Agriculture – CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, 13148-218, São Paulo
[5] ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, UNICAMP, Campinas, 13084-971, São Paulo
来源：
Natural Products and Bioprospecting | 2016年 / 6卷 / 4期
基金：
巴西圣保罗研究基金会;
关键词：
Brazilian savanna fruits; Natural fungicides; Phytopathogens; Residues;
D O I：
10.1007/s13659-016-0101-y
中图分类号：
学科分类号：
摘要：
Abstract: The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity. In this study, ethanolic extracts from leaves and fruit residues (peel and seeds) of three Brazilian savanna species (Acrocomia aculeata, Campomanesia adamantium and Caryocar brasiliense) were evaluated against phytopathogenic fungi. Additionally, the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated. Extracts from C. brasiliense (pequi) peel and leaves were active against Alternaria alternata, Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 µg/mL. When incorporated in solid media, these extracts extended the lag phase of A. alternata and A. solani and reduced the growth rate of A. solani. Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid, quinic acid, ellagic acid, glucogalin and corilagin. The oral acute toxicity was relatively low, being considered safe for use as a potential natural fungicide. Graphical Abstract: [Figure not available: see fulltext.]. © 2016, The Author(s).
引用
收藏
页码：195 / 204
页数：9
相关论文
共 38 条
[1]  
Camele I., Altieri L., de Martino L., de Feo V., Mancini E., Rana G.L., Int. J. Mol. Sci., 13, pp. 2290-2300, (2012)
[2]  
Braghini R., Pozzi C.R., Aquino S., Rocha L.O., Correa B., Appl. Radiat. Isot., 67, pp. 1622-1628, (2009)
[3]  
Tian J., Ban X., Zeng H., Huang B., He J., Wang Y., Food Control, 22, pp. 1992-1999, (2011)
[4]  
de Rodriguez D.J., Garcia R.R., Castillo F.D.H., Gonzalez C.N.A., Galindo A.S., Quintanilla J.A.V., Zuccolotto L.E.M., Ind. Crops Prod., 34, pp. 960-966, (2011)
[5]  
Maswada H.F., Abdallah S., J. Biol. Sci., 23, pp. 1698-1705, (2013)
[6]  
Silva F.M., Paula J.E., Espindola L.S., Mycoses, 52, pp. 511-517, (2009)
[7]  
Santos B.R., Paiva R., Dombroski J.L.D., Martinotto C., Nogueira R.C., Silva A.A.N., Bol. Agrop. Univ. Federal de Lavras., 64, pp. 5-33, (2004)
[8]  
Silva M.R., Lacerda D.B.C.L., Santos G.G., Martins D.M.O., Ciênc. Rural., 38, pp. 1790-1793, (2008)
[9]  
Agostini-Costa T.S., da Silva D.B., Vieira R.F., Sano S.M., Ferreira F.R., Frutas nativas da região Centro-Oeste do Brasil, pp. 15-30, (2010)
[10]  
Passos X.S., Santos S.C., Ferri P.H., Fernandes O.F.L., Paula T.F., Garcia A.C.F., Silva M.R.R., Rev. Soc. Bras. Med. Trop., 35, pp. 623-627, (2002)
1234