The Role of P53-Dependent Autophagy in the Regulation of Pluripotent Cell Bevavior

被引：0

作者：

Sutula G.I. ^{[1
]}

Vorobev M.L. ^{[1
]}

Suvorova I.I. ^{[1
]}

机构：

[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg

来源：

Cell and Tissue Biology | 2020年 / 14卷 / 5期

基金：

俄罗斯基础研究基金会;

关键词：

AMPK; apoptosis; autophagy; differentiation; embryonic stem cells; mTOR; p53; pluripotency; Ulk1;

D O I：

10.1134/S1990519X20050089

中图分类号：

学科分类号：

摘要：

Abstract: Embryonic stem cells (ESCs), and their artificial counterparts, induced pluripotent stem cells (iPSCs) give rise to all differentiated cell types in adult organism.Therefore, pluripotent cells are an inexhaustible cell source for regenerative medicine. However, the successful clinical application of ESCs and iPSCs is associated with the risk of teratoma formation after transplantation of their differentiated products. Oncogenic potential is believed to be associated with the preservation of pluripotent cells resistant to differentiation. For unknown reason under mitogenic stimuli these defective cells did not activate the mechanisms of exit from pluripotency and remained undifferentiated. During embryogenesis, there are special mechanisms for eliminating the abnormal cells unsuitable for embryo development, which are massively triggered before gastrulation, the initial stage of cell differentiation into germ layers. It is known that, prior to implantation, autophagy plays a critical role in embryo formation and can be considered as one of the main cellular strategies aimed at large-scale restructuring of intracellular material after fertilization. It can be proposed that unless massive intracellular reorganization of embryonic cells occur effective, such cells will have defective proteostasis, affecting their differentiation potential. Therefore, the high level of apoptosis observed before gastrulation in embryogenesis is associated with the elimination of mutant cells that are not suitable for differentiation. Damaged cells are marked with the activated p53 protein indicating the p53-dependent elimination mechanisms. And, apparently, the mechanism of the p53 activation is associated with damaged cellular proteostasis, regulated by autophagy. Thus, the p53-dependent autophagy can play a key role in determining the fate of pluripotent cells: induction of cell death and/or resistance to differentiation. We have shown that the p53 protein is tightly integrated with autophagy and, under defective proteostasis, p53 effectively induces autophagy-mediated cell death in pluripotent cells. © 2020, Pleiades Publishing, Ltd.

引用

页码：332 / 340

页数：8

共 40 条

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