Simultaneous detection of guanine and adenine in DNA and meat samples using graphitized mesoporous carbon modified electrode

被引:0
作者
Rajendiran Thangaraj
Annamalai Senthil Kumar
机构
[1] Vellore Institute of Technology University,Environmental and Analytical Chemistry Division, School of Advanced Sciences
来源
Journal of Solid State Electrochemistry | 2013年 / 17卷
关键词
Graphitized mesoporous carbon; Simultaneous detection; Guanine; Adenine; DNA;
D O I
暂无
中图分类号
学科分类号
摘要
A graphitized mesoporous carbon modified glassy carbon electrode (GCE/GMC) prepared by drop coating method without any pre-anodization of the underlying GCE or external binder/matrix, has been demonstrated for simultaneous electrochemical oxidation of guanine (G) and adenine (A) at oxidation potentials 0.60 and 0.85 V vs. Ag/AgCl, respectively, in the presence of thymine (T) by differential pulse voltammetric method in pH 7 phosphate buffer solution. Control voltammetric experiments with unmodified GCE, graphite nanopowder and multiwalled carbon nanotube modified electrodes yielded either feeble or with high-background current responses. Interestingly, the GCE/GMC showed highly efficient, stable and well-defined voltammetric signals. Thymine oxidation signal noticed discretely at 1.15 V vs. Ag/AgCl on the GCE/GMC was not influenced for the simultaneous determination of G and A. Constructed DPV calibration graphs were linear in the range of 25–200 and 25–150 μM, respectively, for the G and A. Corresponding detection limit (S/N = 3) values are 0.76 and 0.63 μM. Real sample analyses for the detection of G and A concentrations in calf-thymus DNA (detected [G]/[A] ratio = 0.82), beef brain and beef liver were successfully demonstrated with recovery values ~100 %.
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页码:583 / 590
页数:7
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