Prion protein gene polymorphism in healthy and BSE-affected Slovak cattle

被引：0
作者：
S. Hreško
M. Mojžiš
L’. Tkáčiková
机构：
[1] University of Veterinary Medicine,Institute of Immunology, Department of Microbiology and Immunology
[2] State Veterinary Institute,undefined
来源：
Journal of Applied Genetics | 2009年 / 50卷
关键词：
bovine spongiform encephalopathy; cattle; PCR; polymorphism; prion protein gene;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Variation of thePrP gene was examined in healthy and BSE-affected Slovak cattle. According to previous studies, the 23-bp indel polymorphism is supposed to be associated with higher susceptibility to BSE. We investigated 301 samples from healthy cattle of various Slovak breeds and 24 samples obtained from tissues of BSE-affected cattle in Slovakia. We examined thePrP gene for the 23-bp indel polymorphism in the putative promoter region, 12-bp indel polymorphism in the first intron of thePrP gene, variations in number of octapeptide repeat units, and presence of the silent AAC>AAT transition in codon 192 within the protein-coding region of thePrP gene. Altogether we found 23 different genotypes in the group of healthy cattle and only 6 genotypes in the group of BSE-affected cattle. Comparison of homozygotes for the 23-bp insertion and heterozygotes showed significant differences (P < 0.05) in genotype distribution between the examined groups. Thereby the homozygous insertion genotype at the 23-bp indel polymorphism site in the promoter region of the prion protein gene seems to have a protective effect against BSE.
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页码：371 / 374
页数：3
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