Insights on the impact of deep eutectic solvents on the composition of the extracts from lemon (Citrus limon L.) peels analyzed by a novel RP-LC–QTOF-MS/MS method

被引:0
作者
Natasa P. Kalogiouri
Eirini Palaiologou
Emmanouil Nikolaos Papadakis
Dimitris P. Makris
Costas G. Biliaderis
Ioannis Mourtzinos
机构
[1] Aristotle University of Thessaloniki,Laboratory of Analytical Chemistry, Department of Chemistry, School of Sciences
[2] Aristotle University of Thessaloniki,Department of Food Science and Technology, School of Agriculture
[3] Aristotle University of Thessaloniki,Pesticide Science Laboratory, School of Agriculture
[4] University of Thessaly,School of Agricultural Sciences
来源
European Food Research and Technology | 2022年 / 248卷
关键词
Lemons; Peels; By-products; Deep eutectic solvents; LC–QTOFMS, non-target;
D O I
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中图分类号
学科分类号
摘要
Extraction of polyphenolic compounds from lemon peels using deep eutectic solvents (DES) was investigated. A choline chloride-based DES paired with glycerol was used as the extraction solvent, alternative to conventional ones. Response surface methodology (RSM) was employed to optimize the extraction parameters, namely, the water concentration (CW), the liquid to solid ratio (RL/S) and the duration of the extraction (t) in terms of total phenol content and radical scavenging activity; the conditions that can be recommended as optimum for the recovery of the phenolic compounds were 55% (w/v), 13 mL g−1 and 36 min, respectively. The composition of the obtained extracts under optimum conditions was compared with extracts prepared in ethanol and water. A novel reversed phase ultra-pressure electrospray liquid chromatographic time-of-flight mass spectrometric method (RP-UPLC–ESI–QTOF-MS) was developed and validated for the determination of individual bioactive compounds in the extracts through target, suspect, and non-target screening. The developed RP-UPLC–ESI–QTOF-MS methodology was validated and presented adequately low limits of detection (LODs) and limits of quantification (LOQs) over the ranges 8.64 (quercetin)–30.4 μg L−1 (p-coumaric acid), and 25.9 (quercetin)–91.2 μg L−1 (p-coumaric acid), respectively. The RSD% of the within-day and between-day assays were lower than 3.2, and 9.8, respectively, demonstrating good method precision. Overall, 10 compounds were determined and quantified through target screening, as well as 4 suspect, and 9 non-target compounds were tentatively identified in the extracts by means of suspect and non-target screening, respectively.
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页码:2913 / 2927
页数:14
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