Simultaneous quantitation of sphingoid bases and their phosphates in biological samples by liquid chromatography/electrospray ionization tandem mass spectrometry

被引：0
作者：
Daisuke Saigusa
Kanako Shiba
Asuka Inoue
Kotaro Hama
Michiyo Okutani
Nagisa Iida
Masayoshi Saito
Kaori Suzuki
Tohru Kaneko
Naoto Suzuki
Hiroaki Yamaguchi
Nariyasu Mano
Junichi Goto
Takanori Hishinuma
Junken Aoki
Yoshihisa Tomioka
机构：
[1] Tohoku University,Laboratory of Oncology, Pharmacy Practice and Sciences, Graduate School of Pharmaceutical Sciences
[2] Tohoku University,Department of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences
[3] Tohoku University,Laboratory of Pharmacotherapy, Graduate School of Pharmaceutical Sciences
[4] Tohoku University Hospital,Department of Pharmaceutical Sciences
来源：
Analytical and Bioanalytical Chemistry | 2012年 / 403卷
关键词：
Sphingoid; Sphingoid phosphate; LC/MS/MS; Simultaneous quantitation; Tissue; Plasma;
D O I：
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中图分类号：
学科分类号：
摘要：
We developed a liquid chromatography/electrospray ionization tandem mass spectrometry method for the simultaneous quantitative determination of C18 sphingosine (Sph), C18 dihydrosphingosine (dhSph), C18 phytosphingosine (pSph), C18 sphingosine-1-phosphate (S1P), C18 dihydrosphingosine-1-phosphate (dhS1P), and C18 phytosphingosine-1-phosphate (pS1P). Samples were prepared by simple methanol deproteinization and analyzed in selected reaction monitoring modes. No peak tailing was observed on the chromatograms using a Capcell Pak ACR column (1.5 mm i.d. × 250 mm, 3 μm, Shiseido). The calibration curves of the sphingoids showed good linearity (r > 0.996) over the range of 0.050–5.00 pmol per injection. The accuracy and precision of this method were demonstrated using four representative biological samples (serum, brain, liver, and spleen) from mice that contained known amounts of the sphingoids. Samples of mice tissue such as plasma, brain, eye, testis, liver, kidney, lung, spleen, lymph node, and thymus were examined for their Sph, dhSph, pSph, S1P, dhS1P, and pS1P composition. The results confirmed the usefulness of this method for the physiological and pathological analysis of the composition of important sphingoids.
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页码：1897 / 1905
页数：8
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