In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis F. Flavicarpa

被引：20

作者：

Faria J.L.C. ^{[1
]}

Segura J. ^{[1
]}

机构：

[1] Depto. de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, 46100-Burjasot Valencia, Av. Vicent Andres Estelles s/n

来源：

In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 3期

关键词：

Adventitious buds; Ammonium and nitrate ratios; Ethylene; Passiflora edulis F. flavicarpa;

D O I：

10.1007/s11627-997-0024-8

中图分类号：

学科分类号：

摘要：

Hypocotyl and leaf explants from Passiflora edulis F. flavicarpa were evaluated for morphogenesis when cultured on several nutrient media supplemented with benzyladenine and indoleacetic acid. The effect of silver thiosulfate on growth-regulator-induced morphogenesis was also investigated. Murashige and Skoog medium was more effective than woody plant medium in promoting adventitious bud differentiation. The omission of ammonium or nitrate from the Murashige and Skoog medium and a disequilibrium from the Murashige and Skoog nitrate:ammonium ratio drastically reduced the bud-forming capacity of the explants. The inclusion of silver thiosulfate in the culture medium significantly increased the differentiation and development of adventitious shoots. Regenerated shoots were excised and induced to root on basal Murashige and Skoog medium. Plants were transplanted to pots and grown ex vitro.

引用

页码：209 / 212

页数：3

共 26 条

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