Isolation and characterization of fourteen polymorphic tetranucleotide microsatellites for Shangcheng Stout Salamander (Pachyhynobius shangchengensis)

被引：0
作者：
Hui Wang
Wenbo Shi
Lifeng Zhu
Xia Luo
Zhong Chen
Wenli Xie
Demin Han
Qing Chang
Baowei Zhang
机构：
[1] Anhui University,School of Life Science
[2] Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences
来源：
Conservation Genetics Resources | 2010年 / 2卷
关键词：
Shangcheng Stout Salamander; Microsatellite; Tetranucleotide; Population genetics; Landscape genetics;
D O I：
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学科分类号：
摘要：
We developed 14 highly polymorphic tetranucleotide microsatellites for the Shangcheng Stout Salamander, Pachyhynobius shangchengensis, a salamandrid endemic to the Dabie Mountain in China. Loci were isolated from a genomic library enriched for (ATAG)n repetitive elements and primers were tested on 21–47 individuals. The number of alleles ranged from 5 to 19 per locus, and the observed heterozygosity (HO) ranged from 0.738 to 0.912. These markers could be a useful tool to investigate population genetic structure, levels of gene flow, population differentiation, mating systems, as well as in studies of landscape genetics in Pachyhynobius shangchengensis.
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页码：121 / 123
页数：2
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