Speciation of antimony(III) and antimony(V) in cell extracts by anion chromatography/ inductively coupled plasma mass spectrometry

被引：0

作者：

N. Ulrich

Pninit Shaked

Dan Zilberstein

机构：

[1] University of Hannover,

[2] Department of Inorganic Chemistry,undefined

[3] Callinstr. 9,undefined

[4] 30167 Hannover,undefined

[5] Germany,undefined

[6] University of Haifa,undefined

[7] Department of Biology,undefined

[8] Israel,undefined

来源：

Fresenius' Journal of Analytical Chemistry | 2000年 / 368卷

关键词：

Chromatography; Mass Spectrometry; Detection Limit; Retention Time; Optimum Condition;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

An analytical method for the separation and quantification of Sb(III) and Sb(V) using anion chromatography with ICP-MS is presented. The optimum conditions for the separation of the antimony species were established with 15 mmol/L nitric acid at pH 6 as eluent system on a PRP-X100 column. The retention times for antimony(V) and antimony(III) were 85 s and 300 s with detection limits of 0.06 μg/L and 0.29 μg/L, respectively. The proposed method was applied to cell extracts of Leishmania donovani, which were incubated with antimony(III) and antimony(V). Some metabolism seemed to occur within the cells.

引用

页码：62 / 66

页数：4