Quantitative analysis of biosurfactants using Fourier Transform Infrared (FT-IR) spectroscopy

被引：0
作者：
J. Gartshore
Y.C. Lim
D.G. Cooper
机构：
[1] McGill University,Department of Chemical Engineering
来源：
Biotechnology Letters | 2000年 / 22卷
关键词：
biosurfactants; infrared spectroscopy; quantification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A quick and simple technique has been developed that is suitable to quantify the concentration of most types of biosurfactants in a typical growth medium using infrared spectroscopy. The area of the peak due to the carbonyl bond of the biosurfactant was measured and compared to the area of the nitrile peak in an internal standard (anthracenecarbonitrile). This ratio showed a linear trend with the concentration of biosurfactant in the medium for concentrations from 0.2 to 3.2 g biosurfactant l−1. The presence of hydrocarbons in the media and variations in the pH of the media did not interfere with the measurements.
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页码：169 / 172
页数：3
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