Molecular characterization and assessment of genetic diversity of inbred lines showing variability for drought tolerance in maize

被引：0
作者：
T. Nepolean
Ishwar Singh
Firoz Hossain
Neha Pandey
H. S. Gupta
机构：
[1] Indian Agricultural Research Institute,Maize Genetics Unit, Division of Genetics
[2] Directorate of Maize Research,undefined
[3] PUSA,undefined
来源：
Journal of Plant Biochemistry and Biotechnology | 2013年 / 22卷
关键词：
Genetic diversity; SSRs; Drought; Mapping population; Heterotic grouping; Maize;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A set of 24 genotypes bred at different centres in India as well as in CIMMYT showing variability for drought tolerance were selected for molecular and morpho-physiological characterization. A set of 35 SSR markers, having genome-wide coverage, was chosen for genotyping the inbreds. These markers generated a total of 111 polymorphic alleles with an average of 3.17 alleles per locus. The minimum and maximum PIC value was 0.27 and 0.77 with a mean of 0.5. A total of 13 unique alleles were found in the 24 inbred lines. The coefficient of genetic dissimilarity ranged from 0.192 to 0.803. NJ-based tree suggested the presence of three major clusters of which, two of them had subgroups. Phenotyping of inbreds by morpho-physiological traits revealed that there was a positive relationship among root length, chlorophyll content, relative water content while anthesis-silking interval was negative relationship with all these traits. Genotyping data complemented by morpho-physiological parameters were used to identify a number of pair-wise combinations for the development of mapping population segregating for drought tolerance and potential heterotic pairs for the development of drought tolerant hybrids.
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页码：71 / 79
页数：8
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