In vitro and in planta Interaction Evidence between Nicotiana tabacum Thaumatin-Like Protein 1 (TLP1) and Cucumber mosaic virus Proteins

被引:0
作者
Min Jung Kim
Byung-Kook Ham
Hwa Ran Kim
In-Ju Lee
Young Jin Kim
Ki Hyun Ryu
Young In Park
Kyung-Hee Paek
机构
[1] Korea University,School of Life Sciences and Biotechnology
[2] Seoul Women’s University,Division of Environmental & Life Sciences
来源
Plant Molecular Biology | 2005年 / 59卷
关键词
CMV 1a protein; thaumatin-like protein (TLP); yeast two-hybrid; yeast three-hybrid;
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摘要
Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.
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页码:981 / 994
页数:13
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