Specific downregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells

被引：0
|
作者：
Raquel T Lima
Luís M Martins
José E Guimarães
Clara Sambade
M Helena Vasconcelos
机构：
[1] IPATIMUP — Institute of Molecular Pathology and Immunology of the University of Porto,Department of Microbiology
[2] Cancer Research UK,undefined
[3] Faculty of Medicine of the University of Porto and Hospital S João,undefined
[4] Faculty of Pharmacy of the University of Porto,undefined
来源：
Cancer Gene Therapy | 2004年 / 11卷
关键词：
siRNAs; RNAi; Bcl-2; xIAP;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Antiapoptotic genes such as bcl-2 or xIAP may be responsible for resistance to apoptosis induced by cytotoxic drugs. The aim of this study was to investigate if downregulation of bcl-2 or xIAP by RNA interference (RNAi) would sensitize MCF-7 cells to etoposide and doxorubicin. FITC-siRNAs uptake was verified by fluorescence microscopy and downregulation of Bcl-2 or XIAP was confirmed by Western Blotting. Both siRNAs reduced the number of viable cells and increased cellular apoptosis. Treatment with siRNAs followed by treatment with etoposide or doxorubicin further reduced the number of viable cells, when compared to either of the treatments alone. Therefore, downregulation of bcl-2 or xIAP by RNAi enhances the effects of etoposide and doxorubicin.
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页码：309 / 316
页数：7
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