Characterization of the ATP-binding cassette transporter gene expression profile in Y79: a retinoblastoma cell line

被引：0
作者：
Doris Hendig
Thomas Langmann
Ralf Zarbock
Gerd Schmitz
Knut Kleesiek
Christian Götting
机构：
[1] Universitätsklinik der Ruhr-Universität Bochum,Institut für Laboratoriums
[2] Universität Regensburg, und Transfusionsmedizin, Herz
[3] Universität Regensburg, und Diabeteszentrum Nordrhein
来源：
Molecular and Cellular Biochemistry | 2009年 / 328卷
关键词：
ABC transporter; ABC protein; Gene expression; Retinoblastoma; Y79;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Chemotherapy failure was reported in treatment of retinoblastoma suggesting a role for ATP-binding cassette (ABC) proteins. Little is known about the expression pattern of ABC proteins in this cancer type. We investigated the gene expression profile of 47 ABC proteins in the human retinoblastoma cell line Y79 by TaqMan low-density array. Analysis revealed 31 ABC transporter genes expressed in this tumor cell line. Y79 cells demonstrate high gene expression of ABCA7, ABCA12, ABCB7, ABCB10, ABCC1, ABCC4, ABCD3, ABCE1, ABCF1, ABCF2, and ABCF3 (more than twofold compared to pooled RNA from different tissues). Moreover, we show that Y79 cells exhibit an active calcein efflux pointing to multidrug resistance protein (MRP)-like transporter activity. In summary, we present for the first time an ABC transporter gene expression profile in cells derived from retinoblastoma. Most of the highly expressed ABC transporter genes are typical markers of cancer cells and might exhibit potential targets for medical treatment of retinoblastoma.
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页码：85 / 92
页数：7
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