Expression and purification of ubiquitin-specific protease (UBP1) ofSaccharomyces cerevisiae in recombinantEscherichia coli

被引：0
作者：
Kang-In Na
Myoung-Dong Kim
Won-Ki Min
Jeong-Ah Kim
Woo-Jong Lee
Dae-Ok Kim
Kyung Moon Park
Jin-Ho Seo
机构：
[1] Seoul National University,Department of Agricultural Biotechnology and Center for Agricultural Biomaterials
[2] Korea Institute of Industrial Technology,Korea Biotechnology Commercialization Center
[3] Kyung Hee University,Department of Food Science and technology
[4] Hongik University,Department of Chemical System Engineering
来源：
Biotechnology and Bioprocess Engineering | 2005年 / 10卷
关键词：
ubiquitin-specific protease; UBP1;
D O I：
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摘要：
Truncated form of UBP1, an ubiquitin-specific protease ofSaccharomyces cerevisiae, was overexpressed inEscherichia coli. The hexahistidine residue (His6) was fused to the N-terminus of truncated UBP1 and the corresponding recombinant protein was purified with high yield by immobilized metal affinity chromatography. The truncated form of UBP1 protein was functional to cleave ubiquitinated human growth hormone as substrate. Effects of pH and temperature were investigated in order to optimize deubiquitinating reactions for the truncated UBP1. Optimum temperature and pH for the cleavage reaction were 40°C and pH 8.0, respectively.
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页码：599 / 602
页数：3
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