Inter-monomer cross-linking affects the thermal transitions in F-actin

被引：0
|
作者：
F. Könczöl
D. Lőrinczy
Zs. Vértes
G. Hegyi
J. Belagyi
机构：
[1] University of Pecs,Institute of Forensic Medicine, Faculty of Medicine
[2] University of Pecs,Biophysical Department, Faculty of Medicine, Institute of Biophysics
[3] University of Pecs,Institute of Physiology, Faculty of Medicine
[4] Eötvös University,Department of Biochemistry
来源：
Journal of Thermal Analysis and Calorimetry | 2010年 / 101卷
关键词：
Actin cross-linking; Thermal stability; DSC;
D O I：
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摘要：
Chemical cross-links which covalently connected the Cys-374 and Glu-41 residues of adjacent monomers in the same strand of F-actin were used to follow the consequences of the modification for the motional and structural properties of the actin filaments. DSC measurements reported that the inter-monomer cross-links shifted the thermal transition temperature and affected strongly the cooperativity of the transition in comparison with uncross-linked F-actin. Addition of HMM to F-actin induced significant decrease of the transition temperature to lower value from 69.4 to 67. 5 °C.
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页码：549 / 553
页数：4
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