Maltotriose metabolism by Saccharomyces cerevisiae

被引:0
作者
Claudio R. Zastrow
Marcelo A. Mattos
Claudia Hollatz
Boris U. Stambuk
机构
[1] Universidade Federal de Santa Catarina,Departamento de Bioquímica, Centro de Ci^encias Biológicas
来源
Biotechnology Letters | 2000年 / 22卷
关键词
brewing; maltose; maltotriose; transport;
D O I
暂无
中图分类号
学科分类号
摘要
Saccharomyces cerevisiae grew slower but reached higher cellular densities when grown on 20 g maltotriose l−1 than on the same concentration of glucose or maltose. Antimycin A (3 mg l−1) prevented growth on maltotriose, but not on glucose or maltose, indicating that it is not fermented but is degraded aerobically. This was confirmed by the absence of ethanol and glycerol production. Active uptake of maltotriose across the plasma membrane is the limiting step for metabolism, and the low rate of maltotriose transport observed in maltotriose-grown cells is probably one of the main reasons for the absence of maltotriose fermentation by S. cerevisiae cells.
引用
收藏
页码:455 / 459
页数:4
相关论文
共 50 条
  • [1] Gonçalves PM(1998)Starting up yeast glycolysis Trends Microbiol. 6 314-319
  • [2] Planta RJ(1999)Multiple Appl. Environ. Microbiol. 65 450-456
  • [3] Jespersen L(1995)-glucoside transporter genes in brewer's yeast J. Am. Soc. Brew. Chem. 53 24-29
  • [4] Cesar LB(1979)Improvement of maltose fermentation efficiency: constitutive expression of Mol. Cell. Biochem. 27 139-146
  • [5] Meaden PG(1996) genes in brewing yeasts J. Inst. Brew. 102 19-25
  • [6] Jakobsen M(1989)Energetic irrelevance of aerobiosis for Yeast 5 159-165
  • [7] Kodama Y(1994) growing on sugars Biotechnol. Lett. 16 89-94
  • [8] Fukui N(1999)Mitochondrial relevance to yeast fermentative performance: a review Biochem. Educ. 27 177-180
  • [9] Ashikari T(1998)Kinetics of growth and glucose transport in glucose-limited chemostat cultures of Biochim. Biophys. Acta 1379 118-128
  • [10] Shibano Y(1999) CBS 8066 FEMS Microbiol. Lett. 170 105-110