Chromatographic separation of enantiomers of non-protein α-amino acids after derivatization with Marfey’s reagent and its four variants

被引:0
作者
R. Bhushan
Virender Kumar
Shivani Tanwar
机构
[1] Indian Institute of Technology Roorkee,Department of Chemistry
来源
Amino Acids | 2009年 / 36卷
关键词
Chiral separation; Non-protein α-amino acids; FDNP-; -Ala-NH; FDNP-; -Phe-NH; FDNP-; -Val-NH; FDNP-; -Leu-NH; FDNP-; -Pro-NH; Normal and RPTLC; RPHPLC;
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摘要
Some non-protein α-amino acids were derivatized with 1-fluoro-2,4-dinitrophenyl-5-l-alaninamide (Marfey’s reagent, MR, FDNP-l-Ala-NH2,) and four of its structural variants FDNP-l-Phe-NH2, FDNP-l-Val-NH2, FDNP-l-Leu-NH2 and FDNP-l-Pro-NH2. The resultant diastereomers were separated by normal and reversed phase thin layer chromatography (TLC) and reversed phase HPLC. In normal phase TLC, best resolution was obtained with solvent combination of phenol-water (3:1) while in reversed phase TLC mixtures of acetonitrile with triethylammonium phosphate buffer were found successful for resolution of diastereomers. The separation behavior of diastereomers prepared with different reagents was compared. The diastereomers of most of the amino acids prepared with FDNP-l-Leu-NH2 were best separated while those prepared with FDNP-l-Pro-NH2 failed to separate in most of the cases. The diastereomers were also separated on a reversed phase C8 column with gradient elution using mixture of aqueous-trifluoroacetic acid (TFA) and acetonitrile and with detection at 340 nm. The effects of TFA concentration, flow rate and run time on HPLC separation were studied.
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页码:571 / 579
页数:8
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