XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC

被引：27

作者：

Xiao, Zhihua ^{[1
]}

Wang, Yijun ^{[2
]}

Ding, Hao ^{[1
]}

机构：

[1] Nanchang Univ, Dept Gastroenterol, Affiliated Hosp 2, 1 Minde Rd, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Univ, Clin Med Coll 2, Nanchang 330006, Jiangxi, Peoples R China

来源：

CELL AND BIOSCIENCE | 2019年 / 9卷

基金：

中国国家自然科学基金;

关键词：

XPD; MiR-29a-3p; Cell proliferation and migration; Hepatocellular carcinoma; HEPATOCELLULAR-CARCINOMA; GROWTH; POLYMORPHISMS; EXPRESSION; INDUCTION; INVASION; PATHWAY; XRCC1; MDM2;

D O I：

10.1186/s13578-018-0269-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ObjectiveThe aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells.MethodsThe expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p.ResultsIn this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B.ConclusionsOur data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.

引用

页数：12

共 34 条

[1]

Askin Debbie Fraser, 2003, Neonatal Netw, V22, P5

[2] Prognostic factors in gemcitabine-cisplatin polychemotherapy regimens in pancreatic cancer: XPD-Lys751Gln polymorphism strikes back [J].

Avan, Amir ;

Pacetti, Paola ;

Reni, Michele ;

Milella, Michele ;

Vasile, Enrico ;

Mambrini, Andrea ;

Vaccaro, Vanja ;

Caponi, Sara ;

Cereda, Stefano ;

Peters, Godefridus J. ;

Cantore, Maurizio ;

Giovannetti, Elisa .

INTERNATIONAL JOURNAL OF CANCER, 2013, 133 (04) :1016-1022

[3] Loss of miR-107, miR-181c and miR-29a-3p Promote Activation of Notch2 Signaling in Pediatric High-Grade Gliomas (pHGGs) [J].

Catanzaro, Giuseppina ;

Sabato, Claudia ;

Russo, Michele ;

Rosa, Alessandro ;

Abballe, Luana ;

Besharat, Zein Mersini ;

Po, Agnese ;

Miele, Evelina ;

Bellavia, Diana ;

Chiacchiarini, Martina ;

Gessi, Marco ;

Peruzzi, Giovanna ;

Napolitano, Maddalena ;

Antonelli, Manila ;

Mastronuzzi, Angela ;

Giangaspero, Felice ;

Locatelli, Franco ;

Screpanti, Isabella ;

Vacca, Alessandra ;

Ferretti, Elisabetta .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (12)

[4] miR-29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF-A [J].

Chen, Ling ;

Xiao, Hong ;

Wang, Zong-Hua ;

Huang, Yi ;

Liu, Zi-Peng ;

Ren, Hui ;

Song, Hang .

BMB REPORTS, 2014, 47 (01) :39-44

[5] miRNAs affect the development of hepatocellular carcinoma via dysregulation of their biogenesis and expression [J].

Chu, Rui ;

Mo, Guangquan ;

Duan, Zhijun ;

Huang, Mei ;

Chang, Jiuyang ;

Li, Xiaodong ;

Liu, Pixu .

CELL COMMUNICATION AND SIGNALING, 2014, 12

[6] Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIH [J].

Coin, F ;

Marinoni, JC ;

Rodolfo, C ;

Fribourg, S ;

Pedrini, AM ;

Egly, JM .

NATURE GENETICS, 1998, 20 (02) :184-188

[7] XPD could suppress growth of HepG2.2.15 and down-regulate the expression of hepatitis B virus x protein through P53 pathway [J].

Ding, Hao ;

Xu, Jiang-jing ;

Huang, Yuan ;

Du, Fang-teng ;

Zhang, Ji-xiang .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 419 (04) :761-767

[8] Association of XPD and XRCC1 Genetic Polymorphisms with Hepatocellular Carcinoma Risk [J].

Guo, Lian-Yi ;

Jin, Xu-Peng ;

Niu, Wei ;

Li, Xiao-Fei ;

Liu, Bao-Hai ;

Wang, Yu-Lin .

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2012, 13 (09) :4423-4426

[9] Inhibiting MDM2-p53 Interaction Suppresses Tumor Growth in Patient-Derived Non-Small Cell Lung Cancer Xenograft Models [J].

Hai, Josephine ;

Sakashita, Shingo ;

Allo, Ghassan ;

Ludkovski, Olga ;

Ng, Christine ;

Shepherd, Frances A. ;

Tsao, Ming-Sound .

JOURNAL OF THORACIC ONCOLOGY, 2015, 10 (08) :1172-1180

[10] Long non-coding RNA H19 regulates E2F1 expression by competitively sponging endogenous miR-29a-3p in clear cell renal cell carcinoma [J].

He, Haowei ;

Wang, Nana ;

Yi, Xiaoming ;

Tang, Chaopeng ;

Wang, Dong .

CELL AND BIOSCIENCE, 2017, 7

← 1 2 3 4 →