Antioxidant activity of Moringa oleifera seed extracts

被引：31

作者：

Jahan I.A. ^{[1
]}

Hossain M.H. ^{[1
]}

Ahmed K.S. ^{[1
]}

Sultana Z. ^{[2
]}

Biswas P.K. ^{[1
]}

Nada K. ^{[1
]}

机构：

[1] Chemical Research Division, BCSIR Laboratories Dhaka, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka

[2] Pharmacy Department, Noakhali Science and Technology University, Noakhali

来源：

Oriental Pharmacy and Experimental Medicine | 2018年 / 18卷 / 4期

关键词：

Antioxidant activity; DPPH; ABTS; Flavonoid; M. oleifera seed kernel; Nitric oxide free radical scavenging; Phenolic; Reducing power;

D O I：

10.1007/s13596-018-0333-y

中图分类号：

学科分类号：

摘要：

The present research evaluates the phytochemical and antioxidant activity of Moringa oleifera Lam. seed kernel grown in Bangladesh. M. oleifera seed kernel was extracted with methanol, acetone and water individually. The phytochemical content was evaluated through the determination of total phenolic, total flavonoid and total tannin contents. In vitro antioxidant capacity was determined following four complementary methods: DPPH (2,2-diphenyl-1-picrylhydrazyl, ABTS{2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)}, and NO (nitric oxide) free radical scavenging and reducing power tests. The total phenolic contents 60.99 ± 0.153, 30.78 ± 0.101 and 90.97 ± 0.134 mg gallic acid eq./g dry extract, total flavonoids contents 10.13 ± 0.171, 13.32 ± 0.101 and 221.76 ± 0.221 mg quercetin eq./g and total tannins contents 97.10 ± 0.153, 73.91 ± 0.107 and 21.74 ± 0.086 mg gallic acid eq./g dry extract were found to be present in methanol, acetone and water extracts respectively. Among the three extracts, the water extract exhibited significant activities for scavenging DPPH, ABTS and NO free radicals with EC50 values 36.89 ± 0.154, 13.20 ± 0.049 and 217.95 ± 0.327 μg/mL respectively against the standard antioxidant compound ascorbic acid and butylated hydroxy anisole. The results of this research revealed that among the three extracts of M. oleifera seed kernel the water extract exhibited significant free radical scavenging activity. Reducing power, total phenolic and total flavonoid contents were also observed to be significant for the water extract. So in contrast of all the results of this study it can be concluded that among the three extracts (methanol, acetone and water) the water extract possessed potent antioxidant activity which support the use of M. oleifera seed kernel as natural antioxidant. © 2018, Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature.

引用

页码：299 / 307

页数：8

共 48 条

[1]

Ahmed K.S., Banik R., Hossain H., Jahan I.A., Vitamin C l-ascorbic acid) content in different parts of Moringa oleifera grown in Bangladesh, Am Chem Sci J, 11, 1, pp. 1-6, (2016)

[2]

Aliev G., Palacios H.H., Lipsitt A.E., Fischbach K., Lamb B.T., Obrenovich M.E., Morales L., Gasimov E., Bragin V., Nitric oxide as an initiator of brain lesions during the development of Alzheimer disease, Neurotox Res, 16, pp. 293-305, (2009)

[3]

Al-Malki A.L., Haddad A., Rabey E.L., The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats, Biomed Res Int, (2015)

[4]

Anwar F., Latif S., Ashraf M., Gilani A.H., Moringa oleifera: a food plant with multiple medicinal uses, Phytother Res, 21, pp. 17-25, (2007)

[5]

Atoui A.K., Mansouri A., Boskou G., Kefalas P., Tea and herbal infusions: their antioxidant activity and phenolic profile, Food Chem, 89, pp. 27-36, (2005)

[6]

Awodele O., Oreagbaa I.A., Odomaa S., Teixeira da Silva J.A., Osunkaluc V.O., Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae), J Ethnopharmacol, 139, 2, pp. 330-336, (2012)

[7]

Bichi M.H., A review of the applications of Moringa oleifera seeds extract in water treatment, Civ Environ Res, 3, pp. 1-9, (2013)

[8]

Blokhina O., Virolainen E., Fagerstedt K., Antioxidants, oxidative damage and oxygen deprivation stress: a review, Ann Bot, 91, pp. 179-194, (2003)

[9]

Chang C., Yang M., Wen H., Chern J., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal, 10, 3, pp. 178-182, (2002)

[10]

Chuang P.H., Lee C.W., Chou J.Y., Murugan M., Shieh B.J., Chen H.M., Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam, Bioresour Technol, 98, 1, pp. 232-236, (2007)

← 1 2 3 4 5 →