Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique

被引：35
作者：
Felleisen R.S.J. [1 ]
机构：
[1] Institute of Parasitology, University of Berne, CH-3012 Berne
来源：
Parasitology Research | 1997年 / 84卷 / 2期
关键词：
Genetic Analysis; Species Level; Squirrel Monkey; Protozoan Parasite; Taxonomic Classification;
D O I：
10.1007/s004360050374
中图分类号：
学科分类号：
摘要：
The taxonomic classification within the genus Tritrichomonas is a subject of controversy, and, potentially, separation of the tritrichomonads from cattle and swine on the species level is not valid. To tackle this hypothesis we comparatively assessed several isolates of protozoan parasites from the three Tritrichomonas species T. foetus, T. suis, and T. mobilensis by the RAPD (random amplified polymorphic DNA) technique. In this method with 20 different primers, all T. foetus and T. suis isolates resulted in identical genomic fingerprints, thus yielding additional evidence for the genetic identity of T. foetus and T. suis. In contrast, it turned out that the species T. mobilensis isolated from the squirrel monkey is genetically distinct and can clearly be discriminated from the other tritrichomonads. Consequently, the results obtained in this study support a possible future revision of the taxonomic classification of the genus Tritrichomonas. © Springer-Verlag 1998.
引用
收藏
页码：153 / 156
页数：3
相关论文
共 2 条
[1]  
Culberson D.E., Pindak F.F., Gardner W.A., Honigberg B.M., Tritrichomonas mobilensis n. sp. (Zoomastigophorea: Trichomonadida) from the Bolivian squirrel monkey Saimiri boliviensis boliviensis, J Protozool, 33, pp. 301-304, (1986)
[2]  
DeCarli G.A., Guerrero J., Comparação antigênica entre Tritrichomonas suis e T. foetus. Immunofluorescência indireta (IFI), Rev Microbiol (Sao Paulo), 6, pp. 55-58, (1975)
1