ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

被引：0
作者：
Lucas Ferraz dos Santos
Eder Jorge de Oliveira
Aline dos Santos Silva
Fabiana Moraes de Carvalho
Juliana Leles Costa
Juliano Gomes Pádua
机构：
[1] Universidade Federal do Recôncavo da Bahia,
[2] Embrapa Mandioca e Fruticultura Tropical,undefined
[3] Faculdade Maria Milza,undefined
[4] Embrapa Recursos Genéticos e Biotecnologia,undefined
来源：
Biochemical Genetics | 2011年 / 49卷
关键词：
Passion fruit; Genetic variability; Molecular markers; Genetic resources;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei’s genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon–Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.
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页码：540 / 554
页数：14
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