Effects of short-term starvation on ghrelin, GH-IGF system, and IGF-binding proteins in Atlantic salmon

被引：0
作者：
E. M. Hevrøy
C. Azpeleta
M. Shimizu
A. Lanzén
H. Kaiya
M. Espe
P. A. Olsvik
机构：
[1] National Institute of Nutrition and Seafood Research (NIFES),Department of Animal Physiology, Faculty of Biology
[2] Complutense University,Computational Biology Unit
[3] Hokkaido University,Department of Biochemistry
[4] Faculty of Fisheries,undefined
[5] University of Bergen,undefined
[6] National Cerebral and Cardiovascular Center Research Institute,undefined
来源：
Fish Physiology and Biochemistry | 2011年 / 37卷
关键词：
Atlantic salmon; Ghrelin; GH-IGF system; IGF-binding proteins; Food deprivation;
D O I：
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学科分类号：
摘要：
The effects of short-time fasting on appetite, growth, and nutrient were studied in Atlantic salmon (Salmo salar) smolts. Feed deprivation did change the energy metabolism with reduced plasma protein and muscle indispensible amino acid levels. Plasma levels of ghrelin were significantly higher in starved salmon compared with fed fish after 2 days, but no differences in circulating ghrelin were found between treatments after 14 days. Two mRNA sequences for ghrelin-1 and ghrelin-2, 430 and 533 bp long, respectively, were detected. In addition, the growth hormone secretagogues-receptor like receptor (GHSR-LR) 1a and 1b were identified. Ghrelin-1 but not ghrelin-2 mRNA levels were affected by starvation in the stomach. Lower ghrelin-1 mRNA levels were detected at day 2 in starved fish compared with fed fish. The mRNA levels of GHSR-LR1a were not affected by starvation. Fasting reduced the phenotypic growth and the transcription of insulin-like growth factor (IGF)-II together with IGF-IIR, but IGF-I mRNA were not regulated in fasted salmon after 14 days. Three IGF-binding proteins (IGFBP) at 23, 32, and 43 kDa were found in salmon, and circulating 23 kDa was significantly increased after 14 days of starvation compared with fed fish, indicating increased catabolism. The levels of IGFBP-1 mRNA were significantly higher in fed and starved fish after 14 days compared to those at the start of the experiment, but no significant difference was observed between the treatments. In conclusion, we have shown that circulating ghrelin and ghrelin-1 mRNA is related to changes in energy metabolism in Atlantic salmon.
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页码：217 / 232
页数：15
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