Intracerebral administration of interleukin-12 (IL-12) and IL-18 modifies the course of mouse scrapie

被引：7

作者：

Pasquali P. ^{[1
]}

Nonno R. ^{[1
]}

Mandara M.T. ^{[2
]}

Di Bari M.A. ^{[1
]}

Ricci G. ^{[2
]}

Petrucci P. ^{[1
]}

Capuccini S. ^{[2
]}

Cartoni C. ^{[1
]}

Macrì A. ^{[1
]}

Agrimi U. ^{[1
]}

机构：

[1] Department of Food Safety and Animal Health, Istituto Superiore di Sanità, 00161, Rome

[2] Department of Biopathological Veterinary Science, Veterinary Medicine School, Università degli Studi di Perugia, Perugia

来源：

BMC Veterinary Research | / 2卷 / 1期

关键词：

Prion Protein; Prion Disease; Brain Homogenate; Scrapie; Untreated Mouse;

D O I：

10.1186/1746-6148-2-37

中图分类号：

学科分类号：

摘要：

Background: Prion diseases are characterised by a neurodegenerative pattern in which the function of immune system remains still elusive. In the present study, we evaluate if an exogenous treatment with Interleukin-12 (IL-12) and IL-18, able to activate microglia, is able to affect scrapie pathogenesis. Results: Cytokines injected intracranially, induced a strong inflammatory response characterised by TNF-α production and microglia activation. Two groups of mice were injected intracerebrally with high dose of ME7 strain of scrapie containing IL-12 and IL-18 or sterile saline. Cytokines-treated mice showed a more pronounced accumulation of PrPSc in brain tissues at 90 days post-inoculation and a shorter mean survival times than untreated mice. Conclusion: We can conclude that intracerebral administration of IL-12 and IL-18 can modulate scrapie pathogenesis possibly through a microglia-mediated pattern. © 2006 Pasquali et al; licensee BioMed Central Ltd.

引用

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