Site-specific integration and tailoring of cassette design for sustainable gene transfer

被引：0

作者：

Angelo Lombardo

Daniela Cesana

Pietro Genovese

Bruno Di Stefano

Elena Provasi

Daniele F Colombo

Margherita Neri

Zulma Magnani

Alessio Cantore

Pietro Lo Riso

Martina Damo

Oscar M Pello

Michael C Holmes

Philip D Gregory

Angela Gritti

Vania Broccoli

Chiara Bonini

Luigi Naldini

机构：

[1] San Raffaele Telethon Institute for Gene Therapy,Division of Regenerative Medicine

[2] Gene Therapy and Stem Cells,Division of Neurosciences

[3] San Raffaele Institute,Division of Regenerative Medicine

[4] Vita-Salute San Raffaele University,undefined

[5] Stem Cell and Neurogenesis Unit,undefined

[6] San Raffaele Institute,undefined

[7] Experimental Hematology Unit,undefined

[8] Gene Therapy and Stem Cells,undefined

[9] Program in Immunology and Bio-immunotherapy of Cancer,undefined

[10] San Raffaele Scientific Institute,undefined

[11] Sangamo BioSciences Inc.,undefined

[12] Present address: Hematopoietic Stem Cell Biology and Differentiation Group,undefined

[13] Department of Differentiation and Cancer Centre for Genomic Regulation,undefined

[14] Barcelona,undefined

[15] Spain.,undefined

来源：

Nature Methods | 2011年 / 8卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Presented is an experimental analysis of the stability of transgene expression, the perturbation of endogenous expression and the perturbation of epigenetic organization upon site-directed delivery of transgenes to the CCR5 and AAVS1 loci in human cells. It provides guidelines for optimal cassette design for stable and nonperturbative gene transfer.

引用

页码：861 / 869

页数：8

共 77 条

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