Evaluation of genotoxicity and oxidative stress of aluminium oxide nanoparticles and its bulk form in Allium cepa

被引：24
作者：
De A. [1 ]
Chakrabarti M. [1 ]
Ghosh I. [1 ]
Mukherjee A. [1 ]
机构：
[1] Department of Botany, Cell Biology and Genetic Toxicology Lab, Centre of Advance Studies, University of Calcutta, 35, Ballygunge Circular Road, Kolkata
来源：
The Nucleus | 2016年 / 59卷 / 3期
关键词：
Chromosome aberration; Comet assay; Cytotoxicity; Keywords; Micronucleus; Nanoparticles; Oxidative stress;
D O I：
10.1007/s13237-016-0179-y
中图分类号：
学科分类号：
摘要：
In the present study, we examined the effects of aluminium oxide nanoparticles (Al2O3 NPs) and bulk aluminium oxide (Al2O3) on Allium cepa in terms of genotoxicity and oxidative stress responses under in planta conditions. The concentrations ranged from 1.25 to 5 µM for NP and its bulk form. Results from this investigation indicated steady rise in chromosome aberrations, micronuclei and DNA strand breaks with increase in concentration of the NPs, effects of Al2O3 NPs being significantly higher than those treated with bulk Al2O3. The divisional frequency was affected by the NP treatment. Oxidative damage such as lipid peroxidation and cell death to Allium under Al2O3 NPs treatments was greater than that observed in bulk Al2O3. A significant increase in activity of guiacol peroxidase was observed in accordance with the depletion in catalase activity in both Al2O3 NPs and bulk Al2O3 treated plants as compared to control. In conclusion, the present study indicated that Al2O3 NPs treatment exerted higher genotoxicity and oxidative stress responses on A. cepa than bulk Al2O3 under in planta conditions. © 2016, Archana Sharma Foundation of Calcutta.
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页码：219 / 225
页数：6
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