Chondroitin Sulfate Is Indispensable for Pluripotency and Differentiation of Mouse Embryonic Stem Cells

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作者：
Tomomi Izumikawa
Ban Sato
Hiroshi Kitagawa
机构：
[1] Kobe Pharmaceutical University,Department of Biochemistry
来源：
Scientific Reports | / 4卷
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摘要：
Chondroitin sulfate (CS) proteoglycans are present on the surfaces of virtually all cells and in the extracellular matrix and are required for cytokinesis at early developmental stages. Studies have shown that heparan sulfate (HS) is essential for maintaining mouse embryonic stem cells (ESCs) that are primed for differentiation, whereas the function of CS has not yet been elucidated. To clarify the role of CS, we generated glucuronyltransferase-I-knockout ESCs lacking CS. We found that CS was required to maintain the pluripotency of ESCs and promoted initial ESC commitment to differentiation compared with HS. In addition, CS-A and CS-E polysaccharides, but not CS-C polysaccharides, bound to E-cadherin and enhanced ESC differentiation. Multiple-lineage differentiation was inhibited in chondroitinase ABC-digested wild-type ESCs. Collectively, these results suggest that CS is a novel determinant in controlling the functional integrity of ESCs via binding to E-cadherin.
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[1]  
Evans MJ(1981)Establishment in culture of pluripotential cells from mouse embryos Nature 292 154-156
[2]  
Kaufman MH(1981)Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells Proc. Natl. Acad. Sci. U.S.A. 78 7634-7638
[3]  
Martin GR(2005)Embryonic stem cell differentiation. Emergence of a new era in biology and medicine Genes Dev. 19 1129-1155
[4]  
Keller G(2005)Microenvironmental regulators of tissue structure and function also regulate tumor induction and progression: the role of extracellular matrix and its degrading enzymes Cold Spring Harb. Symp. Quant. Biol. 70 343-356
[5]  
Bissell MJ(2008)Extracellular matrix dynamics in development and regenerative medicine J. Cell Sci. 121 255-264
[6]  
Kenny PA(2008)The growth factor environment defines distinct pluripotent ground states in novel blastocyst-derived stem cells Cell 135 449-461
[7]  
Radisky DC(2009)Abrogation of E-cadherin-mediated cell-cell contact in mouse embryonic stem cells results in reversible LIF-independent self-renewal Stem Cells 27 2069-2080
[8]  
Daley WP(2010)E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation Stem Cells 28 1315-1325
[9]  
Peters SB(2010)A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts Cell Stem Cell 7 51-63
[10]  
Larsen M(2011)E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming EMBO Reports 12 720-726
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