Synthesis of α-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol

被引：0
|
作者：
O. G. Chernyavskaya
N. V. Shishkanova
A. P. Il'chenko
T. V. Finogenova
机构：
[1] G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences,
[2] Pushchino,undefined
[3] Moscow Region,undefined
[4] Russia,undefined
[5] IBPM,undefined
[6] p-t Nauki 5,undefined
[7] Pushchino,undefined
[8] Moscow Region,undefined
[9] 142292 Russia e-mail: finog@ibpm.serpukhov.su Tel.: +7-0967-730990 Fax: +7-095-923-36-02,undefined
来源：
Applied Microbiology and Biotechnology | 2000年 / 53卷
关键词：
Oxygen; Nitrogen; Enzyme; Oxygen Concentration; Thiamine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The ability of yeast to synthesize α-ketoglutaric acid (KGA) from ethanol has been studied. Thiamine-auxotrophic yeasts of different genera and species may be able to produce KGA; the main condition of synthesis is growth limitation by thiamine. Using a model culture, mutant Yarrowia lipolytica N 1, the principal conditions affecting KGA oversynthesis were identified. These were: thiamine concentration in medium and in cells, nitrogen and oxygen concentration in medium, and pH level. A KGA concentration of 49 g/l and a yield from ethanol consumed of 42% were achieved. Based on the results of the analysis of the activities of the key enzymes participating in ethanol metabolism and KGA synthesis, a concept of the mechanism of KGA biosynthesis by Y. lipolytica yeast is suggested and discussed.
引用
收藏
页码：152 / 158
页数：6
相关论文
共 50 条
  • [1] Synthesis of α-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol
    Chernyavskaya, OG
    Shishkanova, NV
    Il'chenko, AP
    Finogenova, TV
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (02) : 152 - 158
  • [2] α-Ketoglutaric acid synthesis by Yarrowia lipolytica yeast and its regulation
    Morgunov, Igor G.
    Kamzolova, Svetlana V.
    CURRENT OPINION IN BIOTECHNOLOGY, 2013, 24 : S77 - S78
  • [3] Characteristic properties of metabolism of the yeast Yarrowia lipolytica during the synthesis of α-ketoglutaric acid from ethanol
    A. P. Ilchenko
    V. Ya. Lysyanskaya
    T. V. Finogenova
    I. G. Morgunov
    Microbiology, 2010, 79 : 450 - 455
  • [4] Characteristic Properties of Metabolism of the Yeast Yarrowia lipolytica during the Synthesis of α-Ketoglutaric Acid from Ethanol
    Ilchenko, A. P.
    Lysyanskaya, V. Ya.
    Finogenova, T. V.
    Morgunov, I. G.
    MICROBIOLOGY, 2010, 79 (04) : 450 - 455
  • [5] α-Ketoglutaric acid production from rapeseed oil by Yarrowia lipolytica yeast
    Svetlana V. Kamzolova
    Igor G. Morgunov
    Applied Microbiology and Biotechnology, 2013, 97 : 5517 - 5525
  • [6] α-Ketoglutaric acid production from rapeseed oil by Yarrowia lipolytica yeast
    Kamzolova, Svetlana V.
    Morgunov, Igor G.
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 97 (12) : 5517 - 5525
  • [7] Chemically assisted microbial production of succinic acid by the yeast Yarrowia lipolytica grown on ethanol
    Svetlana V. Kamzolova
    Alsu I. Yusupova
    Natalia G. Vinokurova
    Nadezhda I. Fedotcheva
    Maria N. Kondrashova
    Tatiana V. Finogenova
    Igor G. Morgunov
    Applied Microbiology and Biotechnology, 2009, 83 : 1027 - 1034
  • [8] Chemically assisted microbial production of succinic acid by the yeast Yarrowia lipolytica grown on ethanol
    Kamzolova, Svetlana V.
    Yusupova, Alsu I.
    Vinokurova, Natalia G.
    Fedotcheva, Nadezhda I.
    Kondrashova, Maria N.
    Finogenova, Tatiana V.
    Morgunov, Igor G.
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 83 (06) : 1027 - 1034
  • [9] Adaptation of the yeast Yarrowia lipolytica to ethanol
    E. N. Biryukova
    A. Yu. Arinbasarova
    A. G. Medentsev
    Microbiology, 2009, 78
  • [10] Enhanced α-ketoglutaric acid production and recovery in Yarrowia lipolytica yeast by effective pH controlling
    Igor G. Morgunov
    Svetlana V. Kamzolova
    Vladimir A. Samoilenko
    Applied Microbiology and Biotechnology, 2013, 97 : 8711 - 8718
12345