Over-expression of OsDREB genes lead to enhanced drought tolerance in rice

被引:0
作者
Jian-Qiang Chen
Xiu-Ping Meng
Yun Zhang
Mian Xia
Xi-Ping Wang
机构
[1] Beijing Normal University,College of Life Sciences
[2] Fudan University,School of Life Sciences
[3] National Center for Molecular Crop Design,undefined
[4] Weiming Kaituo Agriculture Biotech Co.,undefined
[5] Ltd,undefined
来源
Biotechnology Letters | 2008年 / 30卷
关键词
Drought; OsDREB; Rice; Transcription factors;
D O I
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中图分类号
学科分类号
摘要
The DREB transcription factors, which specifically interact with C-repeat/DRE (A/GCCGAC), play an important role in plant abiotic stress tolerance by controlling the expression of many cold or/and drought-inducible genes in an ABA-independent pathway. We have isolated three novel rice DREB genes, OsDREB1E, OsDREB1G, and OsDREB2B, which are homologous to Arabidopsis DREB genes. The yeast one-hybrid assay indicated that OsDREB1E, OsDREB1G, and OsDREB2B can specifically bind to the C-repeat/DRE element. To elucidate the function of respective OsDREB genes, we have stably introduced these to rice by Agrobacterium-mediated transformation. Transgenic rice plants analysis revealed that over-expression of OsDREB1G and OsDREB2B in rice significantly improved their tolerance to water deficit stress, while over-expression of OsDREB1E could only slightly improved the tolerance to water deficit stress, suggesting that the OsDREBs might participate in the stress response pathway in different manners.
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页码:2191 / 2198
页数:7
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