Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

被引：159
作者：
Kumar D. [1 ]
Dhar D.W. [1 ]
Pabbi S. [1 ]
Kumar N. [2 ]
Walia S. [3 ]
机构：
[1] Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi
[2] Microbiology Department, Kurukshetra University, Kurukshetra
[3] Division of Agricultural Chemical, Indian Agricultural Research Institute, New Delhi
来源：
Indian Journal of Plant Physiology | 2014年 / 19卷 / 2期
关键词：
DEAE-Cellulose-11; Phycocyanin; Spirulina;
D O I：
10.1007/s40502-014-0094-7
中图分类号：
学科分类号：
摘要：
In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD). © 2014 The Author(s).
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页码：184 / 188
页数：4
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